CIMMYT’s Research on Maize DH Technology
CIMMYT’s research on maize DH technology led to several process improvements in the maize DH line production like improved haploid induction rates, improved methods of haploid identification and increased success rates in chromosomal doubling. The publications listed below showcase process improvements at different stages of the maize DH line production process.
Reviews/Book chapters
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Chaikam V, & Prasanna, BM (2020). Doubled haploid technology for rapid and efficient maize breeding. In Accelerated Plant Breeding, 1 : 257-292, Springer, Cham.
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Chaikam V, Molenaar W, Melchinger AE, Boddupalli, PM (2019). Doubled haploid technology for line development in maize: technical advances and prospects. Theor Appl Genet 132 (12): 3227-3243
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Prasanna BM, Chaikam V, Mahuku G (2012) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico, D.F.
Peer reviewed publications
Haploid Induction
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Chaikam V, Nair SK, Gowda M, Hindu V, Melchinger AE, Boddupalli PM (2020). Genetic dissection of maternal influence on in vivo haploid induction in maize. The Crop Journal. 8(2):287-298
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Chaikam V, Nair S, Martinez L, Lopez L, Utz HF, Melchinger A, Boddupalli PM (2018). Marker-assisted breeding of improved maternal haploid inducers in maize for the tropical/subtropical regions. Frontiers in plant sci 9:1527
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Nair SK, Molenaar W, Melchinger AE, Boddupalli PM, Martinez L, Lopez LA and Chaikam V (2017) Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize. Theor Appl Genet 130(6):1113-1122
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Hu H, Schrag TA, Peis R, Unterseer S, Schipprack W et al (2016). The genetic basis of haploid induction in maize identified with a novel genome-wide association method. Genetics, 202(4):1267-1276
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Chaikam V, Martinez L, Melchinger A, Schipprack W and Boddupalli PM (2016) Development and validation of red root marker-based haploid inducers in maize. Crop Science, 56(4):1678-1688
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Prigge V, Schipprack W, Mahuku G, Atlin GN, Melchinger AE (2012). Development of in vivo haploid inducers for tropical maize breeding programs. Euphytica. 185(3):481-90
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Prigge V, Xu X, Li L, Babu R, Chen S, Atlin GN, Melchinger AE (2012). New insights into the genetics of in vivo induction of maternal haploids, the backbone of doubled haploid technology in maize. Genetics. 190(2):781-93
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Prigge V, Sánchez C, Dhillon BS, Schipprack W, Araus JL, Bänziger M, Melchinger AE(2011). Doubled haploids in tropical maize: I. Effects of inducers and source germplasm on in vivo haploid induction rates. Crop science. 51(4):1498-506
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Kebede AZ, Dhillon BS, Schipprack W, Araus JL, Bänziger M, Semagn K, Alvarado G, Melchinger AE (2011). Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize. Euphytica. 180(2):219-26
Haploid identification
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Chaikam V, Lopez LA, Martinez L, Burgueno J, Boddupalli PM (2017). Identification of in vivo induced maternal haploids in maize using seedling traits. Euphytica 213(8):177
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Chaikam V, Nair SK, Babu R, Martinez L, Tejomurtula J and Boddupalli PM (2015) Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression. Theor Appl Genet 128(1):159-171
Chromosome doubling
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Chaikam V, Gowda M, Martinez L, Ochieng J, Omar HA, Prasanna BM (2020). Improving the efficiency of colchicine-based chromosomal doubling of maize haploids. Plants. 9(4):459
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Chaikam V, Gowda M, Nair SK, Melchinger AE, Boddupalli PM (2019). Genome-wide association study to identify genomic regions influencing spontaneous fertility in maize haploids. Euphytica 215(8):138
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Kleiber D, Prigge V, Melchinger AE, Burkard F, San Vicente F, Palomino G, Gordillo GA(2012). Haploid fertility in temperate and tropical maize germplasm. Crop science. 52(2):623-30