Overview of technology/production process

DH line production involves four major steps:

1. Haploid induction from source germplasm using specialized maize genetic stocks called haploid inducers



2. Identification of haploids at seed stage using anthocyanin marker called R1-nj

3. Artificial chromosomal doubling of haploids at seedling stage using mitotic inhibitor chemicals


4. Production of seed for DH lines from fertile doubled haploids


DH Production Facility

CIMMYT established centralized DH line production facilities for Africa at Kiboko, Kenya. Similar facility is also in operation for Latin America at CIMMYT’s experimental station in Agua fria, Mexico. Both public and private sector organizations involved in maize germplasm development can access the DH production service by signing a DH service agreement.​​